rev:  October 20, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant HUMAN NT-1/BCSF-3

Human NT-1/BCSF-3 (Neurotrophin-1/B-Cell Stimulating Factor-3) is identified as part of the IL-6 family of cytokines. It is most homologous to cardiotrophin-1 and ciliary neurotrophic factor. This recombinant NT-1/BCSF-3 is a 22.4 kDa protein containing 199 amino acid residues.

Catalog#: RDI-4518

Package Size: 10 micrograms $231.00   $219.00/pack 2+

                     Mini-pack:cat#RDI-4518p $94.00/2ug      Bulk quotes on request


Supplied: Lyophilized protein from 0.3X PBS

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug).

Reconstitution: The lyophilized NT-1 is soluble in water and most aqueous buffers. The lyophilized powder should first be quick spun in vial, then reconstituted in water to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored dessicated at -20 DEG C. Reconstituted NT-1 should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Biological Activity: NT-1/BCSF-3 weakly supports chick E8 DRG neurite outgrowth at a concentration of 1.0 ng/ml.

AA Sequence :

MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG

PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN

YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS

IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW

LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.



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