rev:    October 20, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant HUMAN Midkine (MK)

Human Midkine (MK) is a new member of the heparin-binding neurotrophic factor family. MK and PTN (Pleiotrophin) are structural homologs, and are highly conserved among species. MK plays important roles in development and carcinogenesis and has several important effects, including promotion of neurite extension and neuronal survival. Recombinant Midkine is a 13.4kDa protein comprising of 123 amino acid residues.

Catalog#: RDI-4516

Package Size: 20 micrograms $231.00   $219.00/pack 2+

                      Mini-pack:cat#RDI-4516P $94.00/5ug       other sizes on request

Supplied: Lyophilized protein from 0.4X PBS, pH 7.4 with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human MK

Reconstitution: The lyophilized MK is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water, to a concentration of 1ug/ul. Allow to set at least 30 minutes at 4 DEG C. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions (1 week 4 DEG C) or stored at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder, though stable at room temperature for a few weeks is best stored dessicated at -20DEG C. Reconstituted MK should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Reactivity: determined by its ability to chemoattract human neutrophils using a concentration range of 0.1 -10.0ng/ml

AA Sequence :

VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT

CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT

KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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