rev: October 20, 2004
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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
Human PTN (Pleiotrophin) also known as HARP (Heparin affin regulatory peptide)
is a new member of the heparin-binding neurotrophic factor family. PTN and
MK(Midkine) are structural homologs, and are highly conserved among species.
PTN plays an important role in developmental processes and promotion of neurite
extension. Recombinant Pleiotrophin is a 15.4kDa protein, comprising of 136
amino acid residues.
Package Size: 20 micrograms $231.00 $219.00/pack 2+
Supplied: Lyophilized protein f0.4X PBS, pH 7.2 with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human PTN.
Reconstitution: The lyophilized PTN is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water, to a concentration of 1ug/ul. Allow to set at least 30 minutes at 4 DEG C. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at -20 DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.
Storage: The lyophilized powder, though stable at room temperature, is best stored dessicated below 0 DEG C. Reconstituted PTN should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.
Reactivity: Data not yet determined
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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