rev:  October 20, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant HUMAN Osteoprotegrin (OPG/OCIF)

Human OPG (Osteoprotegrin) also known as OCIF (Osteoclastogenesis inhibitory factor), is a member of the TNF receptor superfamily. Recombinant human OPG/OCIF specifically acts on bone tissues and increases bone mineral density and bone volume associated with a decrese of active osteoclast number. This recombinant human OPG is a 19.9kda protein comprising of 175 amino acid residues.

Catalog#: RDI-4514

Package Size: 50 micrograms $231.00    $219.00/pack 2+

                       Mini-pack:cat#RDI-4514P $94.00/10ug      Bulk quotes on request

Supplied: Lyophilized protein from 10mM Tris pH7.5 + 75mM NaCl with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human OPG.

Reconstitution: The lyophilized OBG is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in 5mM Tris pH7.5, pH7.5 to a concentration of 1ug/ul. Allow to set at least 30 minutes at 4 DEG C. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder, though stable at room temperature, is best stored dessicated below 0 DEG C. Reconstituted OPG should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Reactivity: The biological activity is determined by its ability to neutralize the stimulation of U937 cells treated with 10ng/ml of soluble RANKL   (sRANKL).

AA Sequence:

METFPPKYLH YDEETSHQLL CDKCPPGTYL KQHCTAKWKT

VCAPCPDHYY TDSWHTSDEC LYCSPVCKEL QYVKQECNRT

HNRVCECKEG RYLEIEFCLK HRSCPPGFGV VQAGTPERNT

VCKRCPDGFF SNETSSKAPC RKHTNCSVFG LLLTQKGNAT

HDNICSGNSE STQK

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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