rev: October 20, 2004
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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
Ciliary Neurotropic Factor(CNTF) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro. More recently, CNTF has been shown to promote survivability and differentiation of other neuronal cell types. This recombinant CNTF is a 22.9 kDa protein containing 200 amino acid residues.
Package Size: 20 micrograms $231.00 $219.00/pack 2+
Mini-pack:cat#RDI-4513P $94.00/5ug Bulk quotes on request
Supplied: Lyophilized protein from 5mM NaP, pH7.5 & 5mM NaCl, with no additives.
Source: E. Coli
Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human CNTF.
Reconstitution: The lyophilized CNTF is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in 5mM sodium phosphate, pH7.5 to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. For most in-vitro applications, CNTF exerts its biological activity in the concentration range of 0.01 to 10.0 ng/ml.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.
Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored dessicated at -20 DEG C. Reconstituted CNTF should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.
Reactivity: The ED50 as determined by the dose dependent proliferation of human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of greater than 500K units/mg.
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Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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