rev:    May 13, 2005

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human Neurturin

Neurturin is a disulfide-linked homodimer neurotrophic factor structurally related to GDNF, Artemin, and Persephin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Neurturin signals through a multicomponent receptor system, composed of RET and one of four GFR alpha (alpha1-alpha4) receptors. Neurturin promotes the development and survival of sympathetic and sensory neurons by signaling through a receptor system composed of RET and GFRalpha2. The functional form of human Neurturin is a disulfide-linked homodimer, of two 11.8 kDa polypeptide monomers (206 total amino acid residues). Each monomer contains seven conserved cysteine residues, one of which (Cys 69) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

Catalog#: RDI-4511


Package Size: 20 micrograms $231.00/package   $219.00/package 2+

                      Mini-Pack:cat#RDI-4511p $94.00/2ug      Bulk quotes on request

Supplied: Lyophilized protein from sterile filtered 10mM NaCitrate pH 4.0 No additives.

Source: E. Coli

Purity: >98% by SDS-Page and FPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human Neurturin

Reconstitution: The lyophilized human Neurturin is soluble in sterile DI water . The material can be reconstituted to a maximum concentration of 1mg/ml in water. Allow to set at least 30 minutes at 4 DEG C. Tighten cap, mix gently by inversion. If desired, can centrifuge for 1-2 minutes at 1000RPM to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored desiccated at -20 DEG C. Reconstituted Neurturin should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Reactivity: This recombinant human Neuturin can be used at a concentration of 100ng/ml to support the survival of 65% newborn rat sympathetic neurons. continued on back page

AA Sequence:

MARLGARPCG LRELEVRVSE LGLGYASDET VLFRYCAGAC

EAAARVYDLG LRRLRQRRRL RRERVRAQPC CRPTAYEDEV

SFLDAHSRYH TVHELSAREC ACV


Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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