rev:   January 18, 2005

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human GDNF

DATA SHEET:recombinant Human GDNF

GDNF is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRalpha (alpha1-alpha4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson's disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer, of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which (Cys 101) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.


Catalog#: RDI-4510

Batch#

Package Size: 10 micrograms $231.00/package  $219.00/package 2+

                        Mini-PacK:cat#RDI-4510p $94.00/2ug       Bulk quotes on request

Supplied: Lyophilized protein from sterile filtered 10mM NaCitrate, 150mM NaCl. No additives.

Source: E. Coli

Purity: >98% by SDS-Page and FPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human GDNF

Reconstitution: The lyophilized human GDNF is soluble in water and most aqueous buffers. For optimum dilution, the lyophilized powder should be reconstituted in water to a yield a 0.1-1mg/ml stock. Allow to set at leats 30 minutes. Mix Gently. If desired, can centrifuge 1 minute 1000rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored dessicated at -20 DEG C. Reconstituted GDNF should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Reactivity: The ED50 as determined by the dose-dependent dopamine uptake of rat ventral mesencephalic cultures was found to be in the range of 5- 10ng/ml

AA Sequence:

MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR

GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY

DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV

YHILRKHSAK RCGCI


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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