rev:  October 20, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human NT-4

Neurotrophin-4 (NT-4/NT-5) is a potent neutrophic factor which supports the growth and survivability of nerve and/or glial cells. The active form of human NT-4 is a dimer, formed by two identical 130 amino acid subunits, which is held together by strong hydrophobic interactions.|

Catalog#: RDI-4504

Package Size: 10 micrograms $235.00/pack  $219.00/pack 2+  Other sizes quoted on request

                      mini-pack cat#RDI-4504P $94.00/2ug      Bulk quotes on request


Supplied: Lyophilized protein with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis. Enodtoxin <0.1ng per ug (1EU/ug)

Reconstitution: The lyophilized human NT-4 is soluble in water and most aqueous buffers. The lyophilized powder hould be reconstituted in water to a concentration of 100 ng/ul.Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions and stored at 4 DEG C ofr up to 1 week or stored at -20 DEG C for future use.
Note:If this material is not to be used for coating orlabeling (or other applications requiring carrier free material), the addition of 0.1% BSA of dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder, though stable at room temperature for a few weeks , but is best stored dessicated at -20 DEG C. Reconstituted NT-4 should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.


Reactivity: The ED50 as determined by the dose dependent induction of choline acetyl transferase activity in RAT Basal Forebrain Septal Cultures was found to be 20-50ng/ml.

AA Sequence:

GVSETAPASR RGELAVCDAV SGWVTDRRTA VDLRGREVEV

LGEVPAAGGS PLRQYFFETR CKADNAEEGG PGAGGGGCRG

VDRRHWVSEC KAKQSYVRAL TADAQGRVGW RWIRIDTACV

CTLLSRTGRA

Precautions: For In vitro research Use Only. Not for use in or   on humans or animals or for diagnostics.


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