rev:     November 9, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  :recombinant Human APOA-1

ApoA-I is a 29.0 kDa protein produced in the liver and intestine, and secreted as the predominant constituent of nascent high-density lipoprotein (HDL) particle. ApoA-I, which is foundexclusively in HDL, has a unique ability to capture and solubilize free cholesterol. This apoA-I ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is though to inhibit atherogenesis. For this reason HDL is also known as the good cholesterol. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I (called apoA-I Milano). The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases. This recombinant human ApoA-I is a 28.2 kDa protein of 244 amino acid residues.

Catalog#: RDI-3511    

Package Size:     100ug $250.00        $219.00/package 2+

                             Mini-Pack: cat#RDI-3511p $94.00/20ug

Supplied: Lyophilized powder with no additives. Endotoxin level is less than 0.1ng (1EU/ug) per ug .

Source: E. Coli

Purity: >97% by SDS-Page and HPLC analysis

Reconstitution:The lyophilized protein should be reconstituted in sterile DI water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes (suggest tightening cap and rotating. Centrifuge 1000 rpm for 1 minute to concentrate material in vial to pull any liquid of sides of vial). This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to one week or at -20 DEG C for future use.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted APOA-1 should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: not determined

AA Sequence:

MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG

KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET

EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV

EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL

RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH

LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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