rev:    October 20, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET:recombinant VIRAL MIP-2

Viral MIP-2 has been shown to inhibit monocyte migration in MIP-1 alpha. Recombinant Viral MIP-2 is a 7.9 kDa protein consisting of 70 amino acid residues.

Catalog#: RDI-3503

Package Size: 50 micrograms $250.00      $219.00/pack 2+

                        Mini-Pack: cat#RDI-3503p $94.00/10ug

Supplied: The sterile filtered solution was lyophilized with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of protein

Reconstitution: Lyophilized MIP-2 should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. -Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or HSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted MIP-2 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by the inhibitory effect on monocyte migration response to human MIP-1 alpha using a concentration range of 1.0µg - 10.0µg/ml of viral MIP-2 will inhibit 25ng/ml of human MIP-1 alpha.

AA Sequence: LGASWHRPDK CCLGYQKRPL PQVLLSSWYP TSQLCSKPGV

                         IFLTKRGRQV CADKSKDWVK KLMQQLPVTA

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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