rev:    October 20, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:     recombinant HUMAN MIP-3 BETA

-Description:Human macrophage inflammatory protein-3B (MIP-3B,  Exodus-3, ELC) is a member of the B-chemokine (C-C) family of  cytokines. It plays an important role in the inflammatory response of human T cells and tissue macrophages. The Human MIP-3B is an 8.8 kDa protein containing 77 amino acid residues

Catalog#: RDI-3229

Package Size: 20 ug/vial $231.00       $219.00/package 2+

                        Mini-Pack:cat#RDI-3229P $94.00/5ug       Bulk quotes on request


Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/ug) of MIP-3 BETA

Reconstitution: The lyophilized MIP-3 BETA should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally stock concentrations of 10ug/ml or greater should be maintained.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted MIP-3B should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by its ability to chemoattract human T cells using a concentration range of 100.0ng/ml.

AA Sequence :

GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC

APPDQPWVER IIQRLQRTSA KMKRRSS

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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