rev:     November 3, 2004

Return (RDI HOME PAGE)

Return (human  cytokine index)


Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human NP-1 (Alpha defensin)

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins which include NP-1, NP-2 and NP-3, are distinguished from the beta-defensins by the pairing of their three disulfide bonds. In addition to antimicrobial activity, NP-1 exhibits chemotactic activity on dendritic cells. NP-1 is expressed as the C-terminal portion of an inactive precursor protein, which also contains a 19 amino acid N-terminal signal sequence and a 45 amino acid polypeptide. NP-1 contains a six-cysteine motif that forms three intra-molecular disulfide bonds. This recombinant human NP-1 is a 3.4 kDa protein containing 30 amino acid residues.


Catalog#: RDI-3142

Package Size: 20 micrograms $231.00/package $219.00/package 2+

                      Mini-Pack:cat#RDI-3142p $94.00/2ug

Supplied: Sterile filtered and lyophilized protein with no additives.


Source: E. Coli

Purity: >95% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human0 NP-1

Reconstitution: The lyophilized human NP-1 is soluble in sterile DI water . The material can be reconstituted to a maximum concentration of 1mg/ml in water. Allow to set at least 30 minutes at 4 DEG C. Tighten cap, mix gently by inversion. If desired, can centrifuge for 1-2 minutes at 1000RPM to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to one week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder, though stable at room temperature for a few weeks but is best stored desiccated at -20 DEG C. Reconstituted NP-1 should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Sequence: ACYCRIPACI AGERRYGYCI YQGRLWAFCC

Reactivity: This recombinant human NP-1 can be used at between 1-10ng/ml to   chemoattract immature dendritic cells.


Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Return (RDI HOME PAGE)

Return (human  cytokine index)


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

Ordering terms

all rights reserved by any/all copyright holders?