rev:    October 20, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant HUMAN TL-1A

Human TL-1A belongs to the TNF superfamily of ligands. It is expressed predominantly in endothelial cells, and to a lesser extent in placenta, lung, kidney, skeletal muscle, pancreas, small intestine and colon. TL-1A inhibits endothelial cell proliferation and angiogenesis, and has been shown to induce NFkB activation, caspase activity, and apoptosis in responding cell lines. TL-1A interacts with TNFRSF25/DR3 receptor, but can also bind to a decoy receptor TNFRSF21/DR6. This recombinant human TL-1A is a 22.0 kDa polypeptide of 194 amino acid residues.

Catalog#: RDI-3123    lot#

Package Size: 20 micrograms $231.00/package   $219.00/package 2+

                       Mini-Pack:cat#RDI-3123p $94.00/2ug

Supplied: Sterile filtered and lyophilized protein with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human TL-1A

Reconstitution: The lyophilized human TL-1A is soluble in sterile DI water . The material can be reconstituted to a concentration of 0.1-1mg/ml in water. Allow to set at least 30 minutes at 4 DEG C. Tighten cap, mix gently by inversion. If desired, can centrifuge for 1-2 minutes at 1000RPM to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to one week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder, though stable at room temperature for a few weeks but is best stored desiccated at -20 DEG C. Reconstituted TL-1A should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Sequence:

Reactivity: Determined by its ability to stimulate IFN-? production by human PBMC using a concentration range of 10.0-100.0 ng/ml. Note: Results may vary with PBMC donors.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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