rev:    October 20, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant HUMAN AITRL (TNFSF18)

-AITRL (activation-induced TNFR member Ligand), the human orthologue of GITRL; is a type II membrane protein belonging to the TNF superfamily. It is expressed on endothelial cells and binds with its receptor, AITR. Interactions of AITRL-AITR are important in regulating T-cell proliferation and TCR-mediated apoptosis. This recombinant human AITRL is a 14.3 kDa protein, comprising the full-length of the TNF-like extracellular domain (126 amino acid residues).

Catalog#: RDI-3122

Package Size: 20 micrograms $231.00   $219.00/package 2+

                     Mini-Pack: cat#RDI-3122p $94.00/5ug

Supplied: Sterile filtered & lyophilized from 10mM Tris pH8.0

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/ug)

Reconstitution: At first use, tighten cap, and gently tap on hard surface or centrifuge 1000rpm for 10 seconds to remove any loss material from cap or sides of vial, Carefully open vial and slowly reconstitute with sterile (endotoxin free) DI water or 10mm tris pH8.0to a maximum concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable for a few weeks at room temperature but it is best stored desiccated at -20 DEG C. Reconstituted material should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Biological Activity : Determined by its ability to stimulate IL-8 production by human PBMC using a concentration range of 1.0-10.0 ng/ml. Note: Results may vary with PBMC donors.

AA Sequence :

ETAKEPCMAK FGPLPSKWQM ASSEPPCVNK VSDWKLEILQ

NGLYLIYGQV APNANYNDVA PFEVRLYKNK DMIQTLYNKS

KIQNVGGTYE LHVGDTIDLI FNSEHQVLKN NTYWGIILLA NPQFIS

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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