rev:     October 20, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant HUMAN 4-1BBR

Human 4-1BB Receptor (4-1BBR) is an inhibitor of 4-1BBL and can block 4-1BBL bioactivity. This recombinant 4-11BBR is a 17.7kDa protein consisting of 167 amino acid residues.

Catalog#: RDI-3115

Package Size: 20 micrograms $231.00   $219.00/pack 2+

                      Mini-pack:cat#RDI-3115p $94.00/5ug


Supplied: Lyophilized protein from 0.3X PBS

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug).

Reconstitution: The lyophilized 4-11BR is soluble in water and most aqueous buffers. The lyophilized powder should first be quick spun in vial, then reconstituted in water to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored dessicated at -20 DEG C. Reconstituted 4-1BBR should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Biological Activity: Determined by its inhibitory effect of the 4-1BBL mediated stimulation of IL-8 production by human PBMC. About 90% of inibition was seen using a concentration of 1µg for both 4-1BBL and 4-1BBR.

AA Sequence :

MERTRSLQDP CSNCPAGTFC DNNRNQICSP CPPNSFSSAG

GQRTCDICRQ CKGVFRTRKE CSSTSNAECD CTPGFHCLGA

GCSMCEQDCK QGQELTKKGC KNCCFGTFND QKRGICRPWT

NCSLDGKSVL VNGTKERDVV CGPSPADLSP GASSVTPPAP

AREPGHS

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.



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