rev:    November 9, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant HUMAN BAFF

-BAFF, a member of the TNF family of ligands, is expressed in T cells, macrophages, monocytes and dendritic cells. BAFF is involved in stimulation of B and T cell function, and is an important survival and maturation factor for peripheral B cells. BAFF signals through three different TNF receptors TACI, BCMA and BAFF-R. The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain. This recombinant human soluble BAFF is a 153 amino acid polypeptide (17.0 kDa), which contains the TNF-like portion of the extracellular domain of BAFF

Catalog#: RDI-3113

Package Size: 20 micrograms $231.00     $219.00/pack 2+      other sizes on request

                        Mini-pack:cat#RDI-3113Xp $94.00/5ug

Supplied: Lyophilized protein from 5mM Tris pH8.0 + 3mM NaCl

Source: E. Coli

Purity: >95% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human BAFF.

Reconstitution: The lyophilized BAFF is soluble in water and most aqueous buffers. The lyophilized powder should first be quick spun in vial, then reconstituted in water to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder, though stable at room temperature, is best stored dessicated at -20 DEG C. Reconstituted BAFF should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Reactivity:

Assay#1: The ED50 as determined by the dose dependent stimulation of IL-8 production by Human PBMC is less than 10 ng/ml. Results may vary with PBMC donors.

Assay#2: Determined by a mouse splenocyte survival assay. Ed50 approx 0.5-2ug/ml

AA Sequence:

MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE

NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC

IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL

KLL

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


-see also  mouse anti-human Baff

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