rev:    October 20, 2004

Return (RDI HOME PAGE)

Return (human cytokine index)


Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant HUMAN TRAIL/Apo2L

Description: Human TRAIL(TNF-Related Apoptosis Inducing Ligand), also called Apo2 Ligand, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apotosis, is achieved through binding to two dealth-signaling receptors, DR4 and DR5. These receptors belong to the TNFR transmembrane family of proteins and contain a cytoplasmic "death-domain" which activates the cells apoptotic machinery. This recombinant Human TRAIL/Apo2 Ligand is a 19.6 kDa protein consisting of 169 amino acid residues and comprises the full length of the TNF-like extracellular domain of Trail.

Catalog#: RDI-3104

Package Size: 50 micrograms $231.00    $219.00/package 2+

                     MINI-PACK: cat#RDI-3104P $94.00/10ug  

                    bulk sizes quoted on request

Supplied: Sterile filtered & lyophilized from 1X PBS No carrier.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/ug) of TRAIL/Apo2L.

Reconstitution: The lyophilized TRAIL/APO2L is soluble in most aqueous buffers. The lyophilized protein should be reconstituted in sterile DI water to a maximum concentration of 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at -20 DEG C for future use. For most in vitro applications, TRAIL exerts its biological activity in the concentration range of 0.2 to 20.0 ng/ml.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder is stable at room temperature but it is best stored desiccated below 0 Deg C. Reconstituted TRAIL should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: TRAIL/Apo2 Ligand is fully biologically active when compared to standards. The ED(50) as determined by the dose dependent stimulation of IL-8 production by Human PBMC is less than 10ng/ml.The induction of apoptosis of LANCaP (human prostate cancer cells by Trail/Apo2L) was typically 2-3 fold higher after 48 hours using 0.1ug/ml.

AA Sequence:

MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS

WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ

EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK

DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG


Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Mouse anti-human Trail/APOII  500ug lyophilized

cat#RDI-TRAILabmXP  $375.00

Rabbit anti-human TRAIL (antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-TRAILabRP   $250.00/50ug

cat#RDI-TRAILabRP1   $375.00/100ug

Rabbit anti-human TRAIL:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-TRAILabRPB      $250.00/25ug

cat#RDI-TRAILabRPB1   $375.00/50ug


Return (RDI HOME PAGE)

Return (human cytokine index)


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

all rights reserved by any/all copyright holders?

Ordering terms