rev:     March 14, 2005

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET::recombinant Human MIP-1 Beta (Macrophage Inflammatory Protein 1 beta)

Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1 alpha and MIP-1 beta are active on human and murine hematopoietic cells. This recombinant human MIP-1 beta is a 7.6 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Catalog#: RDI-309      

Package Size: 10 micrograms  $231.00/PACKAGE    $219.00/Package 2 or more

                         mini-pack cat#RDI-309P $94.00/2ug   inquire for bulk

Supplied: Lyophilized protein with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis and RP-HPLC analysis. Endotoxin level is less than 0.1ng per ug of MIP-1 beta.

Reconstitution: The lyophilized MIP-1B is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water to a concentration of 0.1-1.0 milligram per ml. Allow to set at least 30 minutes at 4 DEG C. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions (1 week 4 DEG C) or stored at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.

Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored desiccated at -20 DEG C. Reconstituted MIP-1beta should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Reactivity: Determined by its ability to chemoattract human monocytes using a concentration range of 5.0- 20.0ng/ml.

AA Sequence :

APMGSDPPTA CCFSYTARKL PHNFVVDYYE TSSLCSQPAV

VFQTKRGKQV CADPSESWVQ EYVYDLELN


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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