rev:  October 18, 2004

Return (RDI HOME PAGE)

Return (human cytokine index)


Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human Stem Cell Factor SCF

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Human SCF is an 18.4 kDa protein containing 164 amino acid residues.

Catalog#: RDI-307

Package Size: 10 micrograms $235.00   $219.00/package 2+

                         Mini-Pack: cat#RDI-307P $94.00/2ug   see bulk below or request quotes


Supplied: Lyophilized protein from 10mM acetic acid with no additives. Endotoxin level is less than 0.1ng per ug (1 EU/microgram) of human SCF.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis.

Reconstitution: Before opening, tighten cap and tap vial on table or quick spin to pull lyophilized powder to bottom of vial. The lyophilized powder should be reconstituted in 10mM acetic acid to a conc of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, rotate, mix well. If desired, centrifuge for 1-2 minutes at 500-1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solution containing carrier protein or medium containing serum. These solutions should be stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. For most in-vitro applications, SCF exerts its biological activity in the concentration range of 0.2 to 20.0 ng/ml.

Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored dessicated at -20 DEG C. Reconstituted SCF should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.


Reactivity: The ED50 as determined by the dose dependent stimulation of TF-1   cells was found to be <2ng/ml. This corresponds to a specific activity   of > 5 X 10 E05  (500K)units/mg.

AA Sequence:

MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV

VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK

KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD

SRVSVTKPFM LPPVA

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


BULK:  Recombinant Human Stem Cell Factor (rSCF) BULK

cat# RDI-307-255X   $5000.00/mg

       RDI-307-255X5  $3450.00/0.5mg

       RDI-307-255S    $250.00/10ug

Description: Recombinant human SCF produced in E.Coli is a single, non-glycosylated polypeptide chain containing 164 amino acids and having a molecular mass of 18409 Dalton. The SCF is purified by proprietary chromatographic techniques.

Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Source: E.Coli

Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rmSCF.

Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing 10mM acetic acide.

Purity: Greater than 98.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Anion-exchange FPLC.

(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be

Met-Glu-GLY-ILE-CYS.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Solubility: It is recommended to reconstitute the lyophilized rSCF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rSCF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rSCF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Biological Activity: rSCF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells eis less then 2ng/ml, corresponding to a Specific Activity of 100K IU/mg.

Protein content: Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of SCF as a Reference Standard.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


see also human SCF and rat SCF and mouse SCF

Return (RDI HOME PAGE)

Return (human cytokine index)


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

Ordering terms

all rights reserved by any/all copyright holders?