rev:  October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human RANTES

RANTES (MCP2) is a protein which has been shown to be a chemoattractant for peripheral blood monocytes. It appears to selectively attract T cells of the CD4+/CD45RO+ phenotype in vitro. HUMAN RANTES is an 7.8Da protein containing 68 amino acid residues.

Catalog#: RDI-306

Package Size: 20 micrograms $231.00   $219.00/pack 2+

                      Mini-Pack: cat#RDI-306P $94.00/5ug

supplied: Lyophilized protein with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1 ng per ug of Rantes.

Reconstitution: The lyophilized RANTES is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. .This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

Storage: The lyophilized powder, though stable at room temperature for a few weeks but is best dessicated at -20 DEG C. Reconstituted RANTES should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Reactivity: Biological activity was determined by a chemotaxis assay using human blood monocytes.chemotactic activity was obtained at a concentration of 1-10 ng/ml.

AA Sequence: SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV

                       FVTRKNRQVC ANPEKKWVRE YINSLEMS

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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