rev:     October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  :recombinant HUMAN Trefoil Factor 2 (TFF2)

-The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF2 has been shown to inhibit gastrointestinal motility and gastric acid secretion. Recent data suggests a potential role for TFF2 in acute and chronic asthma (Nikolaidis, N.M. et al. Am. Journal Respir. Cell Mol. Biol. (2003) 4: 458-464). This Recombinant human TFF2 is a 12.0 kDa polypeptide of 106 amino acid residues, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

Catalog#: RDI-3059 lot#

Package Size: 20 micrograms $231.00     $219.00/pack 2+

                    Mini-Pack: cat#RDI-3059p $94.00/5ug

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of protein

Reconstitution: Lyophilized TFF2 should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or   at -20 DEG C for future use.  

-Note:If this material is not to be used for coating or labeling (or other   applications requiring carrier free material), the addition of 0.1% BSA   or HSA to any further dilution buffers or dilution in sterile culture   media is recommended to enhance stability and minimize absorption to   vial. Ideally, maintain stock >20ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks   but it is best stored desiccated at -20 DEG C. Reconstituted TFF2   should be stored in working aliquots at -20 DEG C. AVOID   FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by its ability to chemoattract human monocytes using   a concentration of 1-10.0ng/ml

AA Sequence :

EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV

TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS

RKCCFSNFIF EVPWCFFPKS VEDCHY


Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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