rev:   October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  :recombinant HUMAN LAG-1

Description:Lymphocyte activation gene-1 protein (LAG-1) ia a CC chemokine and is part of the MIP-1 beta family. Like MIP-1 beta, it shows inflammatory and chemokinetic properties. Lag-1 binds to both CCR5 and to CCR8. Studies have shown that LAG-1 plays an essential role in HIV suppressive factors. This recombinant Human LAG-1 is a 7.7 kDa protein containing 69 amino acid residues.

Catalog#: RDI-3058

Package Size: 20 micrograms $212.00   $200.00/package 2+

                       MINI-PACK:  cat#RDI-3058P $94.00/5ug

                     inquire for bulk

 


Supplied: Sterile filtered & lyophilized . No carrier.

Source: E Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/ug).

Reconstitution: The lyophilized LAG-1 is soluble in most aqueous buffers. The lyophilized protein should be reconstituted in sterile DI water to a maximum concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at 4 DEG C for up to one week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted LAG-1 should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by it's ability to chemoattract CCR5 transfected cells using a concentration of 0.1-10.0 ng/ml

AA Sequence :

APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRGKQV

CADPSESWVQ EYVYDLELN


Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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