rev:    October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET :recombinant Human LD78beta

-Recombinant Human LD78beta is a 7.8 kda protein containing 70 amino acid residues, which is closely related to MIP-1alpha. LD78b has enhanced receptor binding affinities to CCR5 and the beta-chemokine receptorD6 and has been shown to exhibit activities in HIV supression assays.

Catalog#: RDI-3056

Package Size: 20 micrograms $231.00   $219.00/pack 2+

                        Mini-Pack: cat#RDI-3056P $94.00/5ug


Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of protein

Reconstitution: LyophilizedLD78-beta should be reconstituted in water to a concentration of 0.1-1mg/ml. We recommend a quick spin or genlte tap on table top followed by reconstitution in water. Allow to set at least 30 minutes at 4 DEG C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

-Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or HSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted LD78B should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.

AA Sequence :

APLAADTPTA CCFSYTSRQI PQNFIADYFE

TSSQCSKPSV IFLTKRGRQV CADPSEEWVQ

KYVSDLELSA

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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