rev:   October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET :recombinant Human CXCL16

-Recombinant Human CXCL16 is a recently discovered alpha (CXC) chemokine which also has a characteristics of CC chemokines and a structure similar to Fractalkine. It may also play a role in attracting activated lymphocyte subsets during inflammation and may assist immune responses. Recombinant human CXCL16 is a 10.1kda protein containing 89 amino residues.

Catalog#: RDI-3055

Package Size: 25 micrograms $235.00   $219.00/pack 2+

                       Mini-Pack: cat#RDI-3055P $94.00/5ug

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >95% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of protein

Reconstitution: Lyophilized CXCL16 should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

-Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or HSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted CXCL16 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by its ability to chemoattract activated lymphocytes using a concentration range of 1.0-100.0 ng/ml.

Sequence:

NEGSVTGSCY CGKRISSDSP PSVQFMNRLR

KHLRAYHRCL YYTRFQLLSW SVCGGNKDPW

VQELMSCLDL KECGHAYSGI VAHQKHLLP

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


-see also new murine CXCL16

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