rev:   October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET: DATA SHEET:recombinant Human BD-2

-Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. This recombinant Human BD-2 is a 4.3 kDa protein containing 41 amino acid residues.



Catalog#: RDI-3049

Package Size: 20 micrograms $231.00   $219.00/pack 2+

                      Mini-Pack: cat#RDI-3049P  $94.00/5ug

Supplied: Lyophilized powder from 10 mM Acetic Acid with no additives.

Source: E. Coli

Purity: >95% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of protein

Reconstitution: Lyophilized BD-2 should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. -Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or HSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted BD- 2 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by its ability to chemoattract immature human dendritic clles using a concentration range of 10.0-100.0 ng/ml.

AA Sequence: GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P

anti-hBD-2

cat#RDI-BD2abGP $215.00/50ug

cat#RDI-BD2abGP1 $300.00/100ug

Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hBD-2. Anti-hBD-2 specific antibody was purified by affinity chromatography employing immobilized hBD-2 matrix.

anti-hBD-2:Biotin conjugated

-cat#RDI-BD2abGPB $212.00/25ug

cat#RDI-BD2abGPB1 $300.00/50ug

Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hBD-2 (human BD-2). Anti-hBD-2 specific antibody was purified by affinity chromatography and then biotinylated.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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