rev:    October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant HUMAN LEC/NCC-4

-Human LEC (liver-expressed chemokine) also known as NCC-4 is a recently discovered CC chemokine. Human LEC is chemotactic on monocytes. This LEC is a 11.2 kDa protein containing 97 amino acid residues.

Catalog#: RDI-3044

Package Size: 20 micrograms $231.00   $219.00/package 2+

                        mini-pack cat#RDI-3044P  $94.00/5ug

                    -Bulk Quotes upon request

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/ug) of LEC.

Reconstitution: The lyophilized LEC should be reconstituted in water to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at -20 DEG C for future use.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted LEC should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The reactivity of this rhuman LEC was determined by a chemotaxis assay using human monocytes. Maximal chemotaxis was achieved using a concentration of 100ng/ml.

AA Sequence:

QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP

AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST

VKIITAKNGQ PQLLNSQ

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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