rev:    October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant HUMAN GRO-Gamma

-Description:Human GRO-gamma is member of the C-X-C family of chemokines and promotes neutrophil and basophil chemotaxis and degranulation. The rec Gro-gamma is a 7.9 kDa protein containing 73 amino acid residues


Catalog#: RDI-3040

Package Size: 10 micrograms $231.00      $219.00/package 2+

                       mini-pack cat#RDI-3040P  $94.00/2ug

                       -Bulk and other sizes quoted upon request

Supplied: Sterile filtered and then lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/mg) of GRO-gamma

Reconstitution: The lyophilized GRO-gamma should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at 4 DEG C for up to one week or at -20 DEG C for future use.

Storage: The lyophilized powder is stable at room temperature for several weeks but it is best stored desiccated at -20 DEG C. Reconstituted GRO- g should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The biological activity of human GRO-g was determined by a chemotaxis assay using 293 transfected CXCR2 cells. Significant chemotaxis was achieved using a concentration range of 10- 100ng/ml.

AA Sequence :

ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV

IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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