rev:    October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


recombinant Human Hepatocellular Carcinoma (HCC-1)

-Human Hepatocellular Carcinoma (HCC-1) was first isolated from the hemofiltrate of patients with chronic renal failure. HCC-1 has a 46% amino acid sequence identity with MIP-1 alpha/MIP-1Beta and 29%-37% homology with other CC chemokines. This recombinant Human HCC-1 is an 8.4 kda protein containing 72 amino acid residues.

Catalog#: RDI-3038

Package Size: 10 micrograms $231.00    $219.00/pack 2 or more  other sizes on request

                      MINI-PACK cat#RDI-3038p $94.00/2ug

Purity: >98% by SDS-Page and HPLC analyses. Endotoxin level is   less than 0.1ng per ug (1EU/ug) of HCC-1

Reconstitution: The lyophilized human HCC-1 is soluble in water and most aqueous buffers. The material can be reconstituted in water to a concentration of 0.1- 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for future use. Avoid freeze thaw cycles which will result in some loss of activity.

Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DGE C. Reconstituted human HCC-1 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES. EACh FREEZE THAW CYCLE WILL DIMINISH ACTIVITY.

Reactivity: Determined by its ability to chemoattract human monocytes using a concnetration range of 5-20.0ng/ml.

AA Sequence:

TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG

HSVCTNPSDK WVQDYIKDMK EN


For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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