rev:  October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET   :recombinant HUMAN MDC 69   amino acid form

-Human Macrophage-Derived Chemokine (MDC) is a unique member of the CC chemokine family. Human MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial or fibroblast origin. This recombinant version is a peptide of 8.1 kDa that consists of 69 amino acid residues, which contains a glycine and proline at the N-terminus.

Catalog#: RDI-3036A

Package Size: 20 micrograms  $231.00    $219.00/package 2+

                         Mini-Pack:cat#RDI-3036AP $94.00/5ug


Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/mg) of MDC.

Reconstitution: The lyophilized MDC should be reconstituted in water to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder is stable at room temperature for several weeks but it is best stored desiccated at -20 DEG C. Reconstituted MDC should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The reactivity of this rhuman MDC was determined by a chemotaxis assay using human T cells. Significant chemotaxis was achieved using a concentration of 10-100ng/ml.

AA Sequence:

GPYGANMEDS VCCRDYVRYR LPLRVVKHFY WTSDSCPRPG VVLLTFRDKE

ICADPRVPWV KMILNKLSQ


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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