rev:   October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET   :recombinant HUMAN TARC

-Description: Human TARC (thymus and activation regulated chemokine) is a recently discovered protein belonging to the B -chemokine (C-C) family of cytokines. It has been shown to be chemotactic towards T-cells but ineffective on monocytes. Human TARC is an 8.0 kDa protein containing 71 amino acid residues.

Catalog#: RDI-3035

Package Size: 20 micrograms $231.00       $219.00/package 2+

                  Mini-Pack: 5ug cat#RDI-3035P $94.00

               -Bulk Quotes upon request

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >99% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/mg) of TARC

Reconstitution: The lyophilized TARC should be reconstituted in water to a concentration of 0.1-mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder is stable at room temperature but it is best stored desiccated below 0 Deg C. Reconstituted TARC should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The biological activity of human TARC was determined by a chemotaxis assay using human T cells. Significant chemotaxis was achieved using a concentration of 10ng/ml.

AA Sequence:

ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV

FVTVQGRAIC SDPNNKRVKN AVKYLQSLER S


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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