rev:    March 6, 2005

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant Human MIP-4 (PARC)

-MIP-4 is a CC chemokine that is expressed in lymph nodes, lungs, placenta and bone marrow. MIP-4's primary receptor is unknown. MIP-4 chemoattracts lymphocytes, and has been shown to exert activity on both CD4+ and CD8+ T cells. This recombinant human MIP-4 is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines


Catalog#: RDI-3034

Package Size: 10 micrograms $231.00/vial   $219.00/package 2+ BULK ON REQUEST

         

Supplied: Lyophilized protein with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis and HPLC analysis. Endotoxin level is less than 0.1ng per ug of human MIP-4

Reconstitution: The lyophilized MIP-4 is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water or buffer to a concentration of 0.1-1mg/ml. Let sit at least 30 minutes, tighten cap, rotate vial to reconstitute any material on sides of vial. If desired, you cancentrifuge the vial for 1-2 minutes at 1000 rpm to concentrate material in bottom of vial. This material can be diluted into water or other buffered solutions and stored at 4 DEG C for up to 1 week or stored at -20 DEG C for future use. It is recommended that further dilutions of the stock be made into buffer containing carrier protein or media containing serum.

Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored desiccated at -20 DEG C. Reconstituted MIP-4 should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze- THAW Cycles.

Reactivity: The biological of this human MIP-4 was determined by a chemotaxis assay using human T lymphocytes. Significant chemotaxis was achieved at a concentration of 1.0-10ng/ml.

AA Sequence:

AQVGTNKELC CLVTSWQIP QKFLVDYSET SPQCPKPGVI

LLTKRGRQIC ADPNKKWVQK YISDLKLNA

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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