rev:    October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:   recombinant HUMAN EOTAXIN 2

-Eotaxin-2. also known as MPIF-2, is a recently disocvered CC chemokine characterized by its high chemotactic selectivity for eosinophils. This recombinant human Exotaxin-2 is an 8.8kda protein containing 78 amino acid residues.

Catalog#: RDI-3033

Package Size: 20 micrograms $231.00   $219.00/pack 2+

                       Mini-Pack: cat#RDI-3022p $94.00/5ug    inquire for bulk


Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug of eotaxin-2.

Reconstitution: Lyophilized eotaxin-2 should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20DEG C long term for future use. It is recommended that a carrier protein As 0.1% HSA or BSA) or medium serum be added to diluted material.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted exotaxin-2 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by its ability to chemoattract human peripheral blood eosinophils using a concentration of 50.0-100.0ng/ml

AA Sequence:

VVIPSPCCMF FVSKRIPENR VVSYQLSSRS TCLKGGVIFT

TKKGQQFCGD PKQEWVQRYM KNLDAKQKKA SPRARAVA

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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