rev:   October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET   :recombinant HUMAN Cardiotrophin-1

-Human Cardiotrophin-1 is a member of the leukemia inhibitory/ciliary neurotrophic factor/Oncostatin M/interleukin6/interleukin-11 family of cytokines. Cardiotrophin-1 affects cells through interaction with the gp130 receptor subunit. This recombinant version is a peptide of 21.5 kDa that consists of 201 amino acid residues.


Catalog#: RDI-3032

Package Size: 10 micrograms $231.00   $219.00/package 2+

                        Mini-Pack: cat#RDI-3032P $94.00/2ug

                      -Bulk Quotes upon request

Supplied: Lyophilized powder from sterile filtered PBS with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/UG) of cariotrophin-1

Reconstitution: The lyophilized cardiotrophin-1 MUST be reconstituted in 20mM Tris , pH 8.0 (<1mg/ml). Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. Cardiotrophin-1 exerts its biological activity in the concentration of 0.1 to 10ng/ml.

Stability: The lyophilized protein is stable for a few weeks at room temperature, but is best store dessicated at -20 DEG C. Reconstituted cardiotrophin-1 should be stored in working aliquots at -20 DEG C.

BiologicalActivity: The ED50 as determined by the dose dependent proliferation of TF-1 cell is <1ng/ml, correspondimg to a specific activity of > 1million units/mg.

AA Sequence:

MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ

LQGDPFGLPS FSPPRLPVAG LSAPAPSHAG LPVHERLRLD AAALAALPPL

LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP

RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS

A


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the useof this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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