rev:   November 2, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:   recombinant HUMAN Fractalkine(CX3C)

-Description:Human Fractalkine is a member of the newly discovered CX3C family of chemokines which contains both chemokine and mucin domain. The Human Fractalkine is an 8.5 kDa protein containing 76 amino acid residues and comprises only the chemokine domain of Fractalkine.

Catalog#: RDI-3031

Package Size: 20 micrograms $231.00   $219.00/package 2+

                      Mini-Pack cat#RDI-3031P $94.00/5ug              -Bulk Quotes upon request

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin level is less than 0.1ng per ug (1EU/mg) of Fractalkine

Reconstitution: The lyophilized Fractalkine should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at-20 Deg C. Reconstituted Fractalkine should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by a chemotaxis assay using human monocytes. Significant chemotaxis was achieved using a concentration of 10ng/ml.

AA Sequence

QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII

LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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