rev:  October 19,2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):

 see also leptin peptide fragment below (new 2/15/97)


- custom leptin analysis -click for more details, see also new recombinant leptin receptors &   Leptin antibodies
DATA SHEET:recombinant HUMAN LEPTIN

-Human Leptin (Anti-Obesity Protein) is the human homologue of murine leptin, the protein product of the ob (obese) ene in mice and seems to be involved in appetite control. The murine ob gene is expressed in adipose tissue and is thought to regulate the body's fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non insulin dependent) diabetes. HUMAN Leptin is a 16 kDa protein containing 146 amino acid residues.


Catalog#: RDI-3027      (NOTE  NEW CATALOG NUMBER!)

Package Size:  1 milligram    $231.00   $219.00/mg  2 or more

Supplied: Lyophilized powder from 0.5mg/ml solution in 10mM   sodium citrate pH 4.0. No additives.


Source: E. Coli

Purity: >95% by SDS-Page analysis and HPLC analysis.

Endotoxin level is less than 0.1ng per ug of human leptin.

Reconstitution: The lyophilized leptin is poorly soluble at neutral pH. We recommend reconsituting the protein in 10mM Na Citrate pH4.0 to a 1mg/ml stock (it is NOT soluble above 2mg/ml). The protein should dissolve within a few minutes (approx 10 minutes). Once the protein is fully solubilized, the pH can be readjusted to the physiological range and the leptin will remain in solution.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted Leptin should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: This HUMAN leptin has been shown to be biologically active by producing significant effects on body weight,food consumption and plasma glucose levels in mouse models. In ob/ob mice that were treated via intraperitoneal injection once daily at a dose of 5ug leptin/gm of body weight for 7 days, significant effects on body weight, food consumption, and plasma glucose controls were observed compared to saline treated controls.


AA Sequence:

MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL

DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND

LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY

STEVVALSRL QGSLQDMLWQ LDLSPGC



Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


LEPTIN FRAGMENTS

Leptin, the product of the obese gene (ob), is a hormone secreted by adipocytes. Administration of recombinant mouse leptin in ob/ob mice-homozygous ob mutants suffering from obesity-corrected both overeating and obesity. Moreover leptin reduced the appetite and weight of mice with diet-induced obesity, and caused significant loss of body weight and fat content in lean mice.
Lit.Y.Zhang et al: Nature 372, 425 (1994)

M.A. Pelleymounter et al,: Science 269, 541 (1995)

J.L. Halaas et al,:Science 269,543 (1995)

L.A.Campfield et al,:Science 269,546 (1995)/

J.D.McGarry;Curr. Biol. 5,1342 (1995)

R.V. Considine et al,; New Engl.J.Med.334, 292 (1996).


cat#RDI-H3424    Leptin (22-56) (human)        $625.00/mg

(Obese Gene Peptide (22-56)   (human)

H-Val-Pro-lle-Gin-Lys-Val-Gin-Asp-Thr-Lys-Thr-Leu-lle-Lys-Thr-lle-Val-Thr-Arg-lle-Asn-Asp-

lle-Ser-His-Thr-Gln-Ser-Val-Ser-Ser-Lys-Gin-Lys-OH


cat#RDI-H3494 Tyr-Leptin (26-39) (human)    1mg $312.00      5mg $938.00

(Tyr-Obese Gene Peptide (26-39)  (human)

H-Tyr-Lys-Val-Gin-Asp-Asp-Thr-Lys-Thr-Leu-lle-Lys-Thr-lle-Val-OH


cat#RDI-H3426 Leptin (93-105) (human) 1mg $188.00   5mg $625.00

(Obese Gene Peptide (93-105)  (human)

H-Asn-Val-lle-Gin-lle-Ser-Asn-Asp-Leu-Glu-Asn-Leu-Arg-OH


cat#RDI-H3492 Leptin(126-140)(human)    1mg $312.00     5mg  $938.00

(Obese Gene Peptide(126-140)  (human)

H-Glu-Thr-Leu-Asp-Ser-Leu-Gly-Gly-Val-Leu-Glu-Ala-Ser-Gly-Tyr-OH


cat#RDI-H3428 Leptin (138-167) (human)      0.5mg $375.00     1mg $625.00

(Obese Gene Peptide (138-167)(human)

H-Ser-Gly-Tyr-Ser-Thr-Glu-Val-Val-Ala-Leu-Ser-Arg-Leu-Gln-Gly-Ser-Leu-Gln-Asp-Met-Leu-Trp-Gln-Leu-Asp-Leu-Ser-Pro-Gly-Cys-OH


cat#RDI-H3432 Leptin (150-167)(human) 1mg $344.00     5mg $875.00

(Obese Gene Peptide (150-167)(human)

H-Leu-Gln-Gly-Ser-Leu-Gln-Asp-Met-Leu-Trp-Gln-Leu-Asp-Leu-Ser-Paro-Gly-Cys-OH


For In Vitro Research Use Only


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