rev:  November 7, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant Human MACROPHAGE COLONY STIMULATING FACTOR

M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. This recombinant human M-CSF is a 36.8 kDa homodimeric protein containing two 159 amino acid polypeptide subunits.


Catalog#: RDI-3025

Package Size: 10 micrograms $235.00    $219.00/package 2 +

                     mini-pack cat#RDI-3025P $94.00/2ug     inquire for bulk

Supplied: Lyophilized protein from 3mM Tris pH8.5 with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug of M-CSF.

Reconstitution: The lyophilized M-CSF is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water to a concentration of 0.1-0.5 mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions and store at 4 DEG C for up to one week or at -20 DEG C for future use. It is recommended that all dilutions of the stock be made into buffer containing carrier proteins or medium containing serum.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >5ug/ml.

Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored dessicated at -20 DEG C. Reconstituted M-CSF should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.
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Reactivity: The Ed50 as determined by the dose dependent stimulation of the proliferation of murine M-NFS-60 cells is <5ng/ml, which corresponds to a specific activity of >2 X 10 E05 units/mg.

AA Sequence:

MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY

LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD

KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ

GHERQSEGS

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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