rev:   October 20, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


recombinant Human MCP-4

-Human macrophage/monocyte chemotactic protein-4 (MCP-4) is a member of the B- chemokine family (C-C) of cytokines. Like eotaxin, MCP-4 is a powerful eosinophil chemoattractant. Additionally, like MCP-3, MCP-4 elicits chemoattraction of monocytes and T lymphocytes. MCP-4 is a peptide of 8.6kDa that consists of 75 amino acid residues.

Catalog#: RDI-3024

Package Size: 20 micrograms $231.00     $219.00/pack 2 or more

                       MINI-PACK cat#RDI-3024p $94.00/5ug      inquire for bulk

Supplied: sterile filtered and then the protein was lyophilized (no additives)

Source: E. Coli

Purity: >95% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug) of MCP-4

Reconstitution: The lyophilized human MCP-4 is soluble in water and most aqueous buffers. The material can be reconstituted in water to a concentration of 0.1- 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for future use. Avoid freeze thaw cycles which will result in some loss of activity.

Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration >50ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted human MCP-4 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES. EACh FREEZE THAW CYCLE WILL DIMINISH ACTIVITY.

Reactivity: Determined by its ability to chemoattract human peripheral blood monocytes using a concentration of 10.0-100.0ng/ml

AA Sequence:

QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI

CADPKEKWVQ NYMKHLGRKA HTLKT


For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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