rev:    February 21, 2005

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human Granulocyte COLONY STIMULATING FACTOR

G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. This recombinant human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues

Catalog#: RDI-3023      lot#

Package Size: 10 micrograms $235.00   $219.00/pack 2+

                        Mini-Pack:cat#RDI-3023P $94.00/2ug     inquire for bulk

Supplied: Lyophilized protein from 5mM sodium citrate pH4.0, no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug of G-CSF.

Reconstitution: The lyophilized G-CSF is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water or 5mM Citate buffer pH4.0 to a concentration of 0.1-1 mg/ml. Allow to set at least 30 minutes at 4 DEG C , Mix well. If desired, centrifuge for 1-2   minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. It is recommneded that all dilutions of the stock be made into buffer containing carrier proteins (as 0.1% BSA or equivalent)or medium containing serum.

Note:If this material is not to be used for coating or labeling (or other aption of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored dessicated at -20 DEG C DEG C. Reconstituted G-CSF should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Reactivity: The Ed50 as determined by the dose dependent stimulation of the proliferation of murine M-NSF- 60 cells is < 0.1ng/ml, which corresponds to a specific activity of >1 X 107 units/mg.

AA Sequence:

TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK

LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS

GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ

MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF

LEVSYRVLRH LAQP

A280nm Extinction coefficient: 15820

abs 0.1%(=1g/l) 0.847


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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