rev:    Ocotber 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human FLT3-LIGAND

-Human FLT3-ligand (flk2-ligand) stimulates the proliferation and colony formation of certain BM precursor cells including CD34+ cells. This rec FLT3-ligand is a soluble 17.6kDa polypeptide containing 155 amino acid residues which comprises the extracellular domain of the transmembrane flt3-ligand protein.

Catalog#: RDI-3019

Package Size: 10 micrograms $235.00   $219.00/package 2 or more

                     Mini-Pack: cat#RDI-3019P $94.00/2ug    inquire for bulk


Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug of flt3-ligand.

Reconstitution: The lyophilized flt-3 ligand should be reconstituted in water or other aqueous buffers to a concentration of 100 nanograms/ul . Allow to set for at least 30 minutes, tighten cap, rotate, and mix well. If desired, centrifuge for 1 minutes at 1000 rpm to concentrate material in vial. This solution should be diluted into buffer containing carrier protein or medium containing serum.

-Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder is stable at room temperature but it is best stored desiccated below 0 Deg C. reconstituted material should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Reactivity: The ED50 as determined by the dose dependent stimulation of   the proliferation of human AML5 cells is <= 1.0ng/ml, corresponding to a   specific activity of > 1million units/mg.

AA Sequence:

MTQDCSFQHS PISSDFAVKI RELSDYLLQD YPVTVASNLQ

DEELCGGLWR LVLAQRWMER LKTVAGSKMQ GLLERVNTEI

HFVTKCAFQP PPSCLRFVQT NISRLLQETS EQLVALKPWI

TRQNFSRCLE LQCQPDSSTL PPPWSPRPLE ATAPTA



Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.



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