rev:   October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant Human Platelet Factor-4 (PF-4)

Human Platelet-4 (PF-4) is an 8 kDa protein containing 70 amino acid residues which is structurally related to human IL-8 and GRO-alpha. PF-4 is a chemoattractant for human fibroblasts and certain other cells.

Catalog#: RDI-3016  or     cat#RDI-3015

Package Size: 20 micrograms $231.00   $219.00/pack 2+

                     Mini-Pack: cat#RDI-3015P $94.00/5ug        -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis . Endotoxin level is less than 0.1ng per ug (1EU/ug) of PF-4

Reconstitution: The lyophilized PF-4 should be reconstituted in water to a concentration of <1mg/ml then adjusted to 20 mM Tris pH7.6. Allow to set at least 30 minutes at 4 Deg C, rotate, mix well. If desired, centrifuge for 1-2 minutes at 500- 1000 rpm to concentrate material in vial .This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at - 20DEG C for future use.

Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.


Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted PF-4 should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by its ability to chemoattract human fibroblasts using a concentration range of 1.0- 10.0ng/ml.

Sequence:

EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ

LIATLKNGRK ICLDLQAPLY KKIIKKLLES

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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