rev:    October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET: recombinant Human Neutrophil Activating Protein-2  (NAP-2)

(NAP-2) -Human Neutrophil Activating Protein-2 (NAP-2) promotes neutrophil chemotaxis and degranulation. Human NAP-2 is an 7.6kDa protein containing 70 amino acid residues.

Catalog#: RDI-3014

Package Size: 10 micrograms $231.00   $219.00/pack 2+

                              -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng/ug (1EU/ug) of NAP-2)

Reconstitution: The lyophilized NAP-2 should be reconstituted in water to a concentration of 0.1- 1mg/ml. Allow to set at least 30 minutes, rotate and mix well. Centrifuge for 1-2 minutes at 1000RPM to concentrate solution in bottom of vial. This solution can be diluted into other buffered solutions and store at 4 DEG C for up to 1 week or stored at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration <10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted NAP-2 should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by its ability to chemoattract human neutrophils using a concentration range of 1.0- 10.0ng/ml.

AA Sequence:

AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL

KDGRKICLDP DAPRIKKIVQ KKLAGDESAD

Precautions: For In vitro research Use Only. Not for use in   or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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