rev:    October 29, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:RECOMBINANT HUMAN IL-29 (Interferon Lambda -1)

IFN lambda-1,-2, and -3 (also known as IL-29, IL-28A and IL-28B respectively) are distantly related to the IL-10 family and Interferons. All three IFN-lambdas use a distinct receptor system composed of an IFN-lambdaR1 subunit (also called CRF2-12) and IL-10R2 subunit (also called CRF2-14). Signaling through this receptor system induces antiviral defense similar but distinct than the type I Interferons. (Kotenko, SV. et al. Nat Immunology 2003 [1] p. 69-77). This recombinant human IFN-lambda 1 is a 19.8 kDa protein containing 178 amino acid residues.

Catalog#: RDI-3002

Package Size: 20 micrograms $231.00     $219.00/pk 2 or more

                       MINI-PACK cat#RDI-3002p $94.00/5ug        -inquire for custom sizes/bulk packaging

Supplied: The sterile filtered solution was lyophilized from PBS (no additives)

Source: E. Coli

Purity: Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Reconstitution: The lyophilized powder is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in sterile water to a concentration of 0.5-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to further dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Maintain stock >10ug/ml.continued on back page

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted human IFN-Lambda-1 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Recombinant human IFN-lambda 1 induces phosphorylation of STAT2.

AA Sequence :

PTSKPTTTGK GCHIGRFKSL SPQELASFKK ARDALEESLK

LKNWSCSSPV FPGNWDLRLL QVRERPVALE AELALTLKVL

EAAAGPALED VLDQPLHTLH HILSQLQACI QPQPTAGPRP

RGRLHHWLHR LQEAPKKESA GCLEASVTFN LFRLLTRDLK

YVADGNLCLR TSTHPEST

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.   It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.



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