rev:  October 19, 2004

Return (RDI HOME PAGE)

Return (human cytokine index)


Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant Human INTERLEUKIN-17

-Interleukin 17 (IL-17)was identified from a CD4+ T cell DNA library. IL-17 can be induced from primary peripheral blood CD4+ T cells upon stimulation. The cytokine exhibits a high degree of amino acid identity with HVS13, an open reading frame from a T lymphotropic Herpesvirus saimiri and with murine CTLA8. Supernatant from cells transfected with IL-17 induced IL-6 and IL-8 production and enhanced the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. IL-17 is a dilsulfide-linked homodimer of two 15.5 kDa subunits, each containing 136 amino acid residues.

Catalog#: RDI-217

Package Size: 25 micrograms  $235.00     $219.00/package 2 or more

                    -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder from PBS with no additives.


Source: E. Coli

Purity: >97% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug of IL-17.

Reconstitution: Lyophilized IL-17 should be reconstituted in water to a concentration of 0.1-1mg/ml.Allow to set at least 30 minutes, rotate or vortex mix. Centrifuge for 1-2 minutes at 1000rpm to concentrate solution in bottom of vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. It is recommended that a carrier protein (0.1% BSA or HSA)or medium serum be added to diluted material.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted IL-17 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The Ed50 as determined by the dose dependent induction of IL-6 in primary human foreskin was found to be approx 2ng/ml corresponidng to a specifiic activity of >500K units/mg.

AA Sequence:

MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT

NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG

CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI

LVSVGCTCVT PIVHHVA

Precautions: For In vitro research Use Only. Not for use in   or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent fringements that might result with the use of or derivation of this product.


see also rec human IL-17B, IL-17D, IL-17E

Return (RDI HOME PAGE)

Return (human cytokine index)


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA  USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

Ordering terms

all rights reserved by any/all copyright holders?