rev:  October 19, 2004

Return (RDI HOME PAGE)

Return (human cytokine index)


Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant Human INTERLEUKIN-16    (see also 121aa form)

-Interleukin 16 (IL-16) also known as lymphocyte chemoattractant factor (LCF), stimulates a migratory response in CD4+ T cells and CD4 + monocytes and eosinophils. In addition, human IL-16 induces expression of the IL-2 receptor by T cells. IL-16 is a 13.5 kDa protein containing 130 amino acid residues.

Catalog#: RDI-216

Package Size: 10 micrograms $235.00    $219.00/pck 2+

                       Mini-Pack#cat#RDI-216P $94.00/2ug

                    -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder from 1mg/ml stock in PBS with no additives.|

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug of IL-16.

Reconstitution: Lyophilized IL-16 should be reconstituted in water to a concentration of 1 ug/ul. Allow to set at least 30 minutes, rotate or vortex mix. Centrifuge for 1-2 minutes at 1000rpm to concentrate solution in bottom of vial. This solution can be diluted into other buffered solutions or stored at -20 DEG C for future use. It is recommended that a carrier protein or medium serum be added to diluted material. For most in- vitro applications, IL-16 exerts its biological activity in the range of 0.1 to 20.0 ng/ml.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder is stable at room temperature but it is best stored desiccated below 0 Deg C. Reconstituted IL-16 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: This product is fully biologically active when compared to standards. human IL-16 produced a significant increase in chemotaxis at 1.4 ng/ml compared to the medium control in an assay using human CD4 + T lymphocytes.

AA Sequence:

MPDLNSSTDS AASASAASDV SVESTAEATV CTVTLEKMSA GLGFSLEGGK

GSLHGDKPLT INRIFKGAAS EQSETVQPGD EILQLGGTAM QGLTRFEAWN

IIKALPDGPV TIVIRRKSLQ SKETTAAGDS

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Return (RDI HOME PAGE)

Return (human cytokine index)


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

Ordering Terms

all rights reserved by any/all copyright holders?