rev:   October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant Human INTERLEUKIN-15

-Interleukin 15 (IL-15) is a potent T-cell growth factor whose biological activity resembles that of IL-2 and which appears to interact predominantly with the B subunit of the IL-2 receptor (IL-2RB). This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. Whereas an antibody to the ALPHA subunit of the IL-2 receptor was able to block IL-2 but not IL-15 in inducing T-cell proliferation, antibodies to IL-2RB consistently inhibit IL-15 more effectively than they inhibit IL-2. Additionally, it has been shown that neutralizing antibodies to Il-2 failed to block IL-15 activity in stimulating T-cell proliferation and induction of LAK cells. These data indicate that IL-15 acts independently of IL-2 and suggest that these two cytokines can act synergistically in stimulating the cellular immune response. Human IL-15 is a 13kDa protein containing 114 amino acid residues.

Catalog#: RDI-215      LOT#03124

Package Size: 10 micrograms $231.00 or   $219.00/pack 2 or more any

                    Mini-Pack: cat#RDI-215P $94.00/2ug

                     -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder from 20mM Tris pH8.0 with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis Endotoxin less than 0.1ng per ug of IL-15.

Reconstitution: Lyophilized IL-15 should be reconstituted in water or other aqueous buffer to a maximum concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C , tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. For most in-vitro applications, IL-15 exerts its biological activity in the range of 0.1 to 10.0 ng/ml.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.

Storage: The lyophilized powder is stable for a few weeks at room temperature but it is best stored desiccated -20 DEG C. Reconstituted IL-15 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The specific activity is greater than 2 X 10 E06 units per milligram as determined by the dose dependent stimulation of CTLL-2 cell proliferation (Ed50=<0.5ng/ml, corresponding to a specific activity of >2 million units per milligram.

AA Sequence:

MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA

MKCFLLELQV ISHESGDTDI HDTVENLIIL ANNILSSNGN

ITESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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