rev:     October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


Recombinant Human Interleukin-12 (CHO CELL)

-Interleukin-12 (IL-12) is a regulatory protein produced by activated B lymphocytes and macrophages. The biological activities of IL-12 include the stimulation of growth of activated CD4 and CD8+T cells and NK cells. Human IL-12 also promotes the development of proinflammatory/ Th1-like CD4+ cells and cytotoxic CD8 T cells. This recombinant Human IL-12 is a 75kda heterodimeric glycoprtein consisiting of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits (total 503 amino acid residues)

batch data   lot#031S96

cat# RDI-212       $231.00/package     $219.00/package 2 or more

                      mini-pack: cat#RDI-212P $94.00/2ug

Package: 10 micrograms

Source: CHO cells (Chinese Hamster Ovarian Cells)

Purity: Greater than 98% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per ug (1EU/ug) of IL-12.

Formulation: Lyophilized from sterile filtered from PBS pH7.2 (no additives)

Reconstitution: The lyophilized human IL-12 is soluble in water and most aqueous buffers. The lyophilized protein should be reconstituted in PBS pH7.2-7.4 to a concentration of 0.2mg/ml. Allow to set at least 30 minutes at 4 DEG C, rotate, mix well. Centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be stored at 4 DEG C for up to 1 week or at -20'C for future use. For most in vitro applications, IL-12 exerts its biological activity in the concentration range of 0.1 to 10.0 ng/ml.

Stability: The lyophilized protein, though stable for a few weeks at room temperature, is best stored desiccated at -20'C. Reconstituted human IL-12 should be stored in working aliquots at -20'C.

Activity: New assay: The ED50 was determined by the inducation of IFN-gamma from NK cells co-stimulated with IL-18 is <1.0ng/ml, corresponidng to a specific activity of  >1 X 10 E06 units/mg

Old assay: Determined by the stimulation of interferon-gamma production by murine splenocytes co-stimulated with IL-12. The Ed50 for the efect is <0.1ng/ml, corresponding to a specific activity of > 1 x 10 (7).

AA Sequence :

p35- Subunit---

RNLPVA TPDPGMFPCL HHSQNLLRAV SNMLQKARQT LEFYPCTSEE IDHEDITKDK TSTVEACLPL ELTKNESCLN SRETSFITNG SCLASRKTSF MMALCLSSIY EDLKMYQVEF KTMNAKLLMD PKRQIFLDQN MLAVIDELMQ ALNFNSETVP QKSSLEEPDF YKTKIKLCIL LHAFRIRAVT IDRVMSYLNA S

p40-Subunit-

IWELKK DVYVVELDWY PDAPGEMVVL TCDTPEEDGI TWTLDQSSEV LGSGKTLTIQ VKEFGDAGQY TCHKGGEVLS HSLLLLHKKE DGIWSTDILK DQKEPKNKTF LRCEAKNYSG RFTCWWLTTI STDLTFSVKS SRGSSDPQGV TCGAATLSAE RVRGDNKEYE YSVECQEDSA CPAAEESLPI EVMVDAVHKL KYENYTSSFF IRDIIKPDPP KNLQLKPLKN SRQVEVSWEY PDTWSTPHSY FSLTFCVQVQ GKSKREKKDR VFTDKTSATV ICRKNASISV RAQDRYYSSS WSEWASVPCS


Usage: For research use only. Not for use in diagnostic or therapeutic procedures.


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