rev:    October 19. 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human INTERLEUKIN-11

-Interleukin-11 (IL-11) is a potent cell growth factor which stimulates the growth and survivability of certain B-cells and T-cells. Human IL-11 is a 19.1 kDa protein consisting of 178 amino acid residues.

Catalog#: RDI-211

Package Size: 10 micrograms $231.00   $219.00/package 2+

                      Mini-Pack: cat#RDI-211p $94.00/2ug

                       -SEE Bulk Below

Supplied: Lyophilized powder with no additives.Endotoxin <0.1ng/ug (1EU/ug) IL-11.

Source: E. Coli

Purity: >98% by SDS-Page analysis

Reconstitution: The lyophilized IL-11 should be reconstituted in 3mM Tris ph 8.0 to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes, rotate and mix well. Centrifuge for 1-2 minutes at 1000RPM to concentrate solution in bottom of vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or long term at -20 DEG C for future use. For most in- vitro applications, IL-11 exerts its biological activity in the range of 0.1 to 10.0 ng/ml.
Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature but it is best stored desiccated below 0 Deg C. Reconstituted IL-11 should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: This product is fully biologically active as measured by the dose dependent stimulation of the proliferation of murine 7TD1 cells . The ED50 was found to be <10ng/ml, yielding a specific activity of at least 100Ku/mg.

AA Sequence:

MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH

NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK

TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG

IRAAHAILGG LHLTLDWAVR GLLLLKTRL


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Recombinant Human Interleukin-11 (IL-11 (rHuIL-11) BULK

cat# RDI-211-214X     $6000.00/mg     $5000/mg 2+

       RDI-211-214X5 $3750.00/0.5mg

      RDI-211-214S $212.00/10ug

       Bulk quotes on request

Description: Recombinant Human IL-11 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 19256.29 Dalton. The rHuIL-11 is purified by proprietary chromatographic techniques.

Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Source: E.Coli

Endotoxin: Less than 0.1 ng/µg (IEU/µg) of IL-11

Formulation: The protein was lyophilized from a 1mg/ml solution in water with no additives

Purity: Greater than 98.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Anion-exchange FPLC.

(c) Analysis by reducing and non-reducing SDS-PAGE   Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be GLY-Pro-Pro-Pro-Gly. N terminal Methionine has been completely removed enzymatically

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Solubility: It is recommended to reconstitute the lyophilized rHuIL-11 in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rHuIL-11 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuIL-3 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze-thaw cycles.

Biological Activity: This rHuIL-11 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of murine 7TD1 was found to be less then 10ng/ml, corresponding to a Specific Activity of 100KU/mg.

Protein content: Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.95 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of IL-11 as a Reference Standard.

For In vitro research Use Only. Not for use in or on humans or animals .  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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