rev:     March 6, 2005

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human INTERLEUKIN-10

-IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNFa. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. Recombinant human IL-10 is an 18.6 kDa protein of 161 amino acid residues.

Catalog#: RDI-210  

Package Size: 10 micrograms $235.00    $219.00/pack 2+

                      MINI-PACK: cat#RDI-210p $94.00/2ug

                       -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder from 3mM Tris pH7.2 with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis and FPLC analysis. Endotoxin level is less than 0.1ng/ug (1EU/ug) of IL-10

Reconstitution: The lyophilized IL-10 should be reconstituted in 3mM Tris pH7.2 to a maximum concentration of 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, rotate, mix well. Centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial.This solution can be diluted into other buffered solutions containing a carrier protein or medium containing serum and stored at 4 DEG C for up to 1 week or long term at -20 DEG C for future use. For most in-vitro applications, IL-10 exerts its biological activity in the range of 0.2 to 20.0 ng/ml.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable for a few weeks at room temperature but it is best stored desiccated at -20 DEG C. Reconstituted IL-10 should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.


Reactivity: This product is fully biologically active as measured by the dose dependent co-stimulation (with murine IL-4) of MC-9 cells. The ED50 was found to be <2 ng/ml, corresponding to a specific activity of >500K units/mg.

AA Sequence:

MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM

KDQLDNLLLL KESLLEDFKG YLGCQALSEM IQFYLEEVMP

QAENQDPDIK AHVNSLGENL KTLRLRLRRC HRFLPCENKS

KAVEQVKNAF NKLQEKGIYK AMSEFDIFIN YIEAYMTMKI RN

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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