rev:   October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human INTERLEUKIN-8

-Interleukin-8 (IL-8) is an 8.9 kDa protein 77 amino acid residues. It promotes neutrophil chemotaxis and degranulation. The 77 amino acid IL-8 is the predominant form secreted by endothelial cells.

Catalog#: RDI-208

Package Size: 25 micrograms $231.00    $219.00/vial 2 or more any combination

                          Mini-Pack: cat#RDI-208p $94.00/5ug

                               -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis and HPLC sequence analysis. Endotoxin level is less than 0.1ng per ug of IL-8.

Reconstitution: The lyophilized IL-8 should be reconstituted in water to a concentration of 0.1-1mg/ml. All to set at least 30 minutes, then mix well. Centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. When possible, addition of BSA or HSA (up to 0.1%) to cytokine solutions diluted below 50ug/ml is advisable). This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or long term at -20 DEG C for future use. For most in-vitro applications, IL-8 exerts its biological activity in the range of 25 to 150 ng/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted IL-8 should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Human IL-8 produces chemotaxis over a wide concentration range in an assay using human peripheral blood neutrophils. Significant chemotaxis was achieved using a concentration range between 25-150ng/ml.

AA Sequence:

AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA

NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.



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