rev:  October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human INTERLEUKIN-7

-Interleukin-7 (IL-7) is a potent lymphoid cell growth factor which affects Pre-B, Pro-B and early T cells. It will also affect mature T cells in combination with other factors such as IL-2. Human IL-7 is a 17.4 kDa protein containing 152 amino acid residues.

Catalog#: RDI-207

Package Size: 10 micrograms $231.00/pack    $219.00/vial 2+

                      Mini-Pack: cat#RDI-207p $94.00/2ug

                   -inquire for custom sizes/bulk packaging


Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis and N-terminal sequence analysis. Endotoxin level is less than 0.1ng per ug of IL-7.

Reconstitution: The lyophilized IL-7 should be reconstituted in water to a maximum concentration of 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, Rotate and mix well. If desired, centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. For most in-vitro applications, IL-7 exerts its biological activity in the range of 0.1 to 10.0 ng/ml.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted IL-7 should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by the dose-dependent stimulation of IXN/2B cells is <0.5ng/ml, corresponding to a specific activity of > 2 X 106 units/mg.

AA Sequence:

MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF

NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL

LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL

KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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